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ming_li
A really tough bot, or a member.
Joined: 08 Aug 2006
Posts: 2
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 Posted: Tue Aug 08, 2006 9:54 am Post subject: biochemical calculations |
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could sumbody pleaze give me some direction with this questn?
I have 20mL of solution of 20mM [14C] pyruvate (50 dpm nmol-1). What would be the specific activity of the pyruvate if you add
(a) 30ml of water
(b)add 11mg of pyruvate (Mr pyruvate is 110)
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RobJim
Senior Member
Joined: 13 Feb 2005
Posts: 320
Location: Los Angeles, CA
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| Posted: Sat Aug 26, 2006 2:25 am Post subject: |
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| Does the [14C] refer to temperature or what? Also, what does dpm mean?
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sdekivit
Regular
Joined: 26 Jul 2005
Posts: 37
Location: Holland
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| Posted: Sat Aug 26, 2006 1:39 pm Post subject: |
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| RobJim wrote: |
| Does the [14C] refer to temperature or what? Also, what does dpm mean? |
it's radioactively labelled pyruvate (pyruvate containing the C-14 isotope). Dpm stands for desintegrations per minute.
the specific activity means the activity per amount of pyruvate. It can be calculated by dividing the total activity by the amount of pyruvate in mg.
So when adding water, there's no change in the specific activity, since the amount of pyruvate doesn't change. Adding unlabelled pyruvate will lower the specifi activity of the pyruvate, since the amount of pyruvate in the solution rises.
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