gorkin
Probably a bot.
Joined: 09 Jun 2006
Posts: 1
|
 Posted: Fri Jun 09, 2006 8:40 am Post subject: Radioligand binding assay |
 |
|
Hi,
Im new here! Having some problems with a radioligand binding assay, namely im wondering whether or not to use a competitive scheme to determine the Kd for a compound from my enzyme. I have relatively little radiolabeled ligand, so I have to use it rather conservatively. So why not use a constant concentration and then add specific amounts of unlabeled ligand? Im not sure if this is acceptable though...Anyone have any suggestions?
thanks.
|
|
